1B0L

RECOMBINANT HUMAN DIFERRIC LACTOFERRIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-05-01
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	156.250, 97.530, 55.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.181
R-free	0.27400
Structure solution method	OTHER
Starting model (for MR)	1lfg
RMSD bond length	0.003
RMSD bond angle	0.010
Data reduction software	DENZO
Data scaling software	CCP4 ((ROTAVATA))
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.046	0.542
Number of reflections	36329
<I/σ(I)>	11.1	1.5
Completeness [%]	82.3	67.8
Redundancy	2.8	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8	277 *	DIALYSIS AGAINST 10MM SODIUM PHOSPHATE PH 8.0, CONTAINING 10% ETHANOL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	80 (mg/ml)
2	1	1	HEPES	10 (mM)
3	1	1		0.5 (M)
4	1	2	sodium phosphate	10 (mM)
5	1	2	ethanol	10 (%(v/v))