1B0L
RECOMBINANT HUMAN DIFERRIC LACTOFERRIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-01 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 156.250, 97.530, 55.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.181 |
| R-free | 0.27400 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1lfg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.010 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.046 | 0.542 |
| Number of reflections | 36329 | |
| <I/σ(I)> | 11.1 | 1.5 |
| Completeness [%] | 82.3 | 67.8 |
| Redundancy | 2.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 8 | 277 * | DIALYSIS AGAINST 10MM SODIUM PHOSPHATE PH 8.0, CONTAINING 10% ETHANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 80 (mg/ml) | |
| 2 | 1 | 1 | HEPES | 10 (mM) | |
| 3 | 1 | 1 | 0.5 (M) | ||
| 4 | 1 | 2 | sodium phosphate | 10 (mM) | |
| 5 | 1 | 2 | ethanol | 10 (%(v/v)) |
