Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | B 1 1 2 |
| Unit cell lengths | 187.800, 72.300, 74.100 |
| Unit cell angles | 90.00, 90.00, 77.60 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fgh |
| RMSD bond length | 0.018 |
| RMSD bond angle | 25.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 30924 | |
| <I/σ(I)> | 4.5 | 1.6 |
| Completeness [%] | 91.8 | 86.8 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 26 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16-18 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris-HCl | 0.25 (M) | |
| 3 | 1 | drop | 0.35 (M) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2.2-2.4 (M) | |
| 5 | 1 | reservoir | bis-Tris-HCl | 0.25 (M) | |
| 6 | 1 | reservoir | 0.35 (M) | ||
| 7 | 1 | drop | ammonium sulfate |
