Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | B 1 1 2 |
Unit cell lengths | 187.400, 72.100, 73.900 |
Unit cell angles | 90.00, 90.00, 77.70 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.162 |
Rwork | 0.162 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fgh |
RMSD bond length | 0.018 |
RMSD bond angle | 25.000 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.630 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 29472 | |
<I/σ(I)> | 7.3 | 2.5 |
Completeness [%] | 88.1 | 79.4 |
Redundancy | 2.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 26 * | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16-18 (mg/ml) | |
2 | 1 | drop | Bis-Tris-HCl | 0.25 (M) | |
3 | 1 | drop | 0.35 (M) | ||
4 | 1 | reservoir | ammonium sulfate | 2.2-2.4 (M) | |
5 | 1 | reservoir | bis-Tris-HCl | 0.25 (M) | |
6 | 1 | reservoir | 0.35 (M) | ||
7 | 1 | drop | ammonium sulfate |