1B0A
5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.520, 84.950, 146.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.000 - 2.560 |
| R-factor | 0.232 * |
| Rwork | 0.218 |
| R-free | 0.29800 * |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.270 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.033 | 0.130 |
| Number of reflections | 12965 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 98.6 | 99.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 * | 4 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 5 (mg/ml) | |
| 2 | 1 | drop | PEG10000 | 13 (%(w/v)) | |
| 3 | 1 | drop | MPD | 1-2 (%) | |
| 4 | 1 | drop | Tris-HCl | 80 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 3 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 80 (mM) |
