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1B0A

5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X12C
Synchrotron siteNSLS
BeamlineX12C
Temperature [K]170
Detector technologyIMAGE PLATE
DetectorMARRESEARCH
Spacegroup nameI 2 2 2
Unit cell lengths64.520, 84.950, 146.140
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution42.000 - 2.560
R-factor0.232

*

Rwork0.218
R-free0.29800

*

Structure solution methodMIR
RMSD bond length0.006
RMSD bond angle1.270

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Refinement softwareX-PLOR (3.8)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.600
High resolution limit [Å]2.5602.560
Rmerge0.0330.130
Number of reflections12965
<I/σ(I)>2.6
Completeness [%]98.699.8
Redundancy33
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

7.8

*

4

*

pH 7.5
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropenzyme5 (mg/ml)
21dropPEG1000013 (%(w/v))
31dropMPD1-2 (%)
41dropTris-HCl80 (mM)
51dropbeta-mercaptoethanol3 (mM)
61reservoirTris-HCl80 (mM)

225158

PDB entries from 2024-09-18

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