1AZZ

FIDDLER CRAB COLLAGENASE COMPLEXED TO ECOTIN

Experimental procedure

Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-05
Detector	RIGAKU RAXIS
Spacegroup name	P 32 2 1
Unit cell lengths	89.110, 89.110, 291.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.189
Rwork	0.189
R-free	0.23800
RMSD bond length	0.011
RMSD bond angle	2.800
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.500
Rmerge	0.095
Total number of observations	109981 *
Number of reflections	41721
Completeness [%]	78.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.5 *	17 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.2 (M)
2	1	reservoir	PEG4000	40 (%)
3	1	reservoir	tricine	0.2 (M)