1AZT
GS-ALPHA COMPLEXED WITH GTP-GAMMA-S
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-06 |
Detector | ADSC QUANTUM |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.300, 96.500, 133.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gia |
RMSD bond length | 0.007 |
RMSD bond angle | 21.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.540 | |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.138 * | |
Number of reflections | 37636 | |
<I/σ(I)> | 8.3 | 2.5 |
Completeness [%] | 94.0 | 95.1 |
Redundancy | 4.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | CRYSTALLIZED IN HANGING DROPS CONTAINING PROTEIN MIXED 1:1 WITH WELL SOLUTION CONSISTING OF 90-100% SATURATED KH2PO4 OR NAH2PO4 (UNBUFFERED)., pH 4.5, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Gsalpha | 12 (mg/ml) | |
2 | 1 | drop | GTPgammaS | 0.8 (mM) | |
3 | 1 | drop | sodium HEPES | 20 (mM) | |
4 | 1 | drop | 5 (mM) | ||
5 | 1 | drop | EDTA | 1 (mM) | |
6 | 1 | drop | dithiothreitol | 5 (mM) | |
7 | 1 | reservoir | potassium phosphate | 90-100 (%sat) | can be replaced by 2.5M sodium phosphate |