1AZO

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	41.600, 72.610, 72.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.208 *
Rwork	0.203
R-free	0.26300
RMSD bond length	0.004 *
RMSD bond angle	1.156 *
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.030 *	0.124 *
Completeness [%]	86.7 *	43.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8 *	20 *	100 MM AMMONIUM ACETATE, 50 MM MAGNESIUM ACETATE, 1 MM DTT, 12-16 % PEG 6000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium acetate	100 (mM)
2	1	reservoir	magnesium acetate	50 (mM)
3	1	reservoir	dithiothreitol	1 (mM)
4	1	reservoir	PEG6000	12-16 (%)