1AZI
MYOGLOBIN (HORSE HEART) RECOMBINANT WILD-TYPE COMPLEXED WITH AZIDE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10-23 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.285, 28.909, 35.874 |
| Unit cell angles | 90.00, 107.16, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.173 * |
| Rwork | 0.170 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.040 |
| Data reduction software | OSCILL |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Rmerge | 0.102 |
| Total number of observations | 14776 * |
| Number of reflections | 5531 |
| Completeness [%] | 71.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 25 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 60 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 65 (%sat) | |
| 7 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 8 | 1 | reservoir | EDTA | 1 (mM) |
