1AZF

CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION

Experimental procedure

Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-08
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	79.140, 79.140, 38.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.193
Rwork	0.193
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lyz
RMSD bond length	0.012
RMSD bond angle	22.270 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.850	2.000
High resolution limit [Å]	1.650	1.650
Rmerge	0.053	0.203
Number of reflections	14036
<I/σ(I)>	11.5
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.6	293 *	pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	85 (mg/ml)
2	1	drop		0.5 (M)
3	1	reservoir	sodium acetate	0.1 (M)