1AZD
CONCANAVALIN FROM CANAVALIA BRASILIENSIS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.290, 71.650, 190.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.24000 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2ctv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.780 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.400 | 3.060 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.150 | 0.500 |
| Total number of observations | 91275 * | |
| Number of reflections | 16811 | |
| <I/σ(I)> | 5.7 | 1.5 |
| Completeness [%] | 90.0 | 88.5 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 2 MICRO-L CBR (10 MG/ML) 2 MICRO-L (11-15% PEG 6000, 0.1 M MES PH 6, pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 50 (mM) | |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | PEG6000 | 11-15 (%) | |
| 6 | 1 | reservoir | MES | 0.1 (M) |
