1AYX

CRYSTAL STRUCTURE OF GLUCOAMYLASE FROM SACCHAROMYCOPSIS FIBULIGERA AT 1.7 ANGSTROMS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	58.140, 87.790, 99.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.144 *
Rwork	0.144
R-free	0.18100
RMSD bond length	0.021
RMSD bond angle	0.036
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000 *
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.042 *
Number of reflections	56654 *
Completeness [%]	99.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.1		50 MM ACETATE BUFFER, PH 5.1, 15 % (W/V) PEG 8000 HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG8000	15-16 (%(w/v))
3	1	drop	acetate	50 (mM)
4	1	reservoir	PEG8000	30-32 (%(w/v))
5	1	reservoir	acetate	100 (mM)