1AYO
RECEPTOR BINDING DOMAIN OF BOVINE ALPHA-2-MACROGLOBULIN
Experimental procedure
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1997-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.570, 107.570, 72.229 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.000 - 1.900 |
| R-factor | 0.1947 |
| R-free | 0.23940 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.619 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 * | 0.265 |
| Total number of observations | 453466 * | |
| Number of reflections | 35876 * | |
| <I/σ(I)> | 23 | 3.9 |
| Completeness [%] | 91.0 * | 82.6 |
| Redundancy | 6.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.9 | 38% PEGMMES 2000, 25 MM CACL2, 100 MM HEPES, PH 6.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | mPEG2000 | 35 (%(w/v)) | |
| 2 | 1 | 1 | HEPES | 100 (mM) | titrated with NaOH |
| 3 | 1 | 1 | 20 (mM) |
