Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 277 |
| Detector technology | FILM |
| Collection date | 1994-04 |
| Detector | FILM |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 362.600, 347.100, 334.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rhv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.700 * |
| Data reduction software | PURDUE (DATA PROCESSING PACKAGE) |
| Data scaling software | PURDUE (DATA PROCESSING PACKAGE) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.130 |
| High resolution limit [Å] | 2.800 | 2.820 |
| Rmerge | 0.110 | 0.182 |
| Number of reflections | 645656 | 101987 * |
| Completeness [%] | 63.1 | 33 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | VIRUS WAS CRYSTALLISED USING THE HANGING DROP METHOD. 5 MICROLITERS OF VIRUS SOLUTION IN 0.25M HEPES BUFFER (PH 7.5), WITH 0.25M NACL WAS MIXED WITH 5 MICROLITERS OF THE RESERVOIR SOLUTION, 0.5-1.5% PEG 8000., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 4-5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 0.25-0.75 (%) | |
| 3 | 1 | reservoir | PEG8000 | 0.5-1.5 (%) | |
| 4 | 1 | reservoir | 5-20 (mM) |
