1AYM
HUMAN RHINOVIRUS 16 COAT PROTEIN AT HIGH RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-04 |
| Detector | FUJI |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 362.600, 347.100, 334.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.150 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR |
| Starting model (for MR) | 2RHN |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 |
| High resolution limit [Å] | 1.900 | 2.140 |
| Rmerge | 0.097 | 0.240 |
| Total number of observations | 3442752 * | |
| Number of reflections | 1452910 | |
| Completeness [%] | 44.5 | 47 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 4-5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 0.25-0.75 (%) | |
| 3 | 1 | reservoir | PEG8000 | 0.5-1.5 (%) | |
| 4 | 1 | reservoir | 5-20 (mM) |
