1AYL
PHOSPHOENOLPYRUVATE CARBOXYKINASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.510, 96.690, 46.960 |
| Unit cell angles | 90.00, 96.63, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23400 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.400 * |
| Data reduction software | WEIS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.076 |
| Number of reflections | 40067 |
| Completeness [%] | 76.7 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.4 * | 21 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PCK | 8 (mg/ml) | |
| 10 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 11 | 1 | reservoir | PEG4000 | 27 (%(w/v)) | |
| 2 | 1 | drop | ATP | 2 (mM) | |
| 3 | 1 | drop | oxalate | 2 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | sodium acetate | 100 (mM) | |
| 7 | 1 | drop | ammonium acetate | 200 (mM) | |
| 8 | 1 | drop | PEG4000 | 12 (%(w/v)) | |
| 9 | 1 | reservoir | sodium acetate | 100 (mM) |
