1AYI
COLICIN E7 IMMUNITY PROTEIN IM7
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-25 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 44.630, 50.677, 75.187 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.178 * |
| Rwork | 0.178 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 10 ALIGNED NMR STRUCTURES OF COLICIN E9 IMMUNITY PROTEIN IM9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.500 |
| Data reduction software | XDS |
| Data scaling software | MARSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.180 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.049 * | 0.156 * |
| Total number of observations | 19772 * | |
| Number of reflections | 5828 | |
| <I/σ(I)> | 14 | 3 |
| Completeness [%] | 93.0 | 87 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 20 * | USING THE HANGING DROP METHOD, PROTEIN (10MG/ML) WAS CRYSTALLISED BY MIXING AN EQUAL VOLUME OF PROTEIN WITH A SOLUTION CONTAINING 55% SATURATED DI-AMMONIUM PHOSPHATE AND 200MM TRIS PH7.2. THE DROP WAS EQUILIBRATED AGAINST A WELL CONTAINING 100% SATURATED DI-AMMONIUM PHOSPHATE AT ROOM TEMPERATURE., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | di-ammonium phosphate | 55 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 200 (mM) | |
| 4 | 1 | reservoir | di-ammonium phosphate | 100 (%sat) |
