1AYF
BOVINE ADRENODOXIN (OXIDIZED)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 44.180, 78.340, 60.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.000 - 1.850 |
| R-factor | 0.195 * |
| Rwork | 0.195 |
| R-free | 0.25300 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.015 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.044 * | 0.204 * |
| Number of reflections | 21775 | |
| <I/σ(I)> | 30.956 | 7.74 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 5 | 5.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.3 * | 4 * | PROTEIN WAS CRYSTALLIZED FROM 30% PEG 4000, 10% GLYCEROL, 100 MM TRIS, PH 7.4, 100MM MGCL2, 20 MG/ML PROTEIN |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 4 | 1 | reservoir | glycerol | 10 (%) | |
| 5 | 1 | reservoir | 100 (mM) |
