1AYC

CRYSTAL STRUCTURES OF PEPTIDE COMPLEXES OF THE AMINO-TERMINAL SH2 DOMAIN OF THE SYP TYROSINE PHOSPHATASE

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	62.200, 62.200, 74.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.189
Rwork	0.189
RMSD bond length	0.012
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.054 *
Total number of observations	43918 *
Number of reflections	6914 *
Completeness [%]	96.0 *	90.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	protein
2	1	reservoir	peptide
3	1	reservoir	PEG4000	20 (%)
4	1	reservoir	MES	100 (mM)