1AY5
AROMATIC AMINO ACID AMINOTRANSFERASE COMPLEX WITH MALEATE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-03-08 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 124.480, 121.440, 55.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.500 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1art |
RMSD bond length | 0.006 |
RMSD bond angle | 23.000 * |
Data reduction software | PROCESS ((RIGAKU)) |
Data scaling software | PROCESS ((RIGAKU)) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 123.000 | 2.800 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.145 |
Total number of observations | 86526 * | |
Number of reflections | 26633 | |
<I/σ(I)> | 14.1 | 5.2 |
Completeness [%] | 97.2 | 96.1 |
Redundancy | 3.3 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.6 | 20 * | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | drop | HEPES-NaOH | 1 (mM) | |
3 | 1 | drop | dithiothreitol | 0.05 (mM) | |
4 | 1 | drop | 0.01 (%) | ||
5 | 1 | drop | PEG4000 | 8.25 (%(w/v)) | |
6 | 1 | drop | sodium acetate | 0.2 (M) | |
7 | 1 | reservoir | PEG4000 | 16.5 (%(w/v)) | |
8 | 1 | reservoir | sodium acetate | 0.4 (M) |