1AY0
IDENTIFICATION OF CATALYTICALLY IMPORTANT RESIDUES IN YEAST TRANSKETOLASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.100, 113.600, 162.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.500 - 2.600 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.26300 |
| Structure solution method | DIFFERENCE FOURIER METHODS |
| Starting model (for MR) | 1trk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.700 |
| High resolution limit [Å] | 2.400 | 2.600 |
| Rmerge | 0.097 | 0.215 |
| Total number of observations | 380321 * | |
| Number of reflections | 36959 | |
| Completeness [%] | 82.7 | 65.1 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | 4 * | Schneider, G., (1989) J. Biol. Chem., 264, 21619. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 7.5 (%) | |
| 3 | 1 | drop | glycylglycine | 25 (mM) | |
| 4 | 1 | drop | thiamine-PP | 2 (mM) | |
| 5 | 1 | reservoir | glycylglycine | 50 (mM) | |
| 6 | 1 | reservoir | thiamine-PP | 2 (mM) |
