1AXZ
ERYTHRINA CORALLODENDRON LECTIN IN COMPLEX WITH D-GALACTOSE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.202, 72.990, 71.290 |
| Unit cell angles | 90.00, 113.44, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.950 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.20600 |
| Structure solution method | DIFFERENCE FOURIER FROM PREVIOUSLY DETERMINED, RELATED STRUCTURE |
| Starting model (for MR) | UNLIGANDED ECORL |
| RMSD bond length | 0.005 |
| RMSD bond angle | 27.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.243 * |
| Total number of observations | 99253 * | |
| Number of reflections | 28798 | |
| <I/σ(I)> | 20 | 4.5 |
| Completeness [%] | 95.4 | 95 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | MPD | 15 (%(v/v)) | |
| 3 | 1 | drop | HEPES | 50 (mM) | |
| 4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 5 | 1 | reservoir | MPD | 30 (%) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | |
| 7 | 1 | reservoir | sodium azide | 0.02 (%) |
