1AXT

IMMUNE VERSUS NATURAL SELECTION: ANTIBODY ALDOLASES WITH THE RATES OF NATURAL ENZYMES

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	97
Detector technology	IMAGE PLATE
Collection date	1996-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	56.500, 65.300, 132.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.150
R-factor	0.2108
R-free	0.31670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cgr
RMSD bond length	0.005
RMSD bond angle	30.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-96

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.065 *	0.302 *
Total number of observations	264288 *
Number of reflections	26432
<I/σ(I)>	25	5.6
Completeness [%]	96.1	95
Redundancy	10	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4		PROTEIN WAS CRYSTALLIZED FROM 18% PEG 4000, 10% ISOPROPANOL, 100 MM HEPES, PH 7.4.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	18 (%)
2	1	1	HEPES	0.1 (M)
3	1	1	isopropanol	10 (%)