1AXN

THE HIGH RESOLUTION STRUCTURE OF ANNEXIN III SHOWS DIFFERENCES WITH ANNEXIN V

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Detector technology	IMAGE PLATE
Collection date	1994-04-13
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	42.544, 69.085, 50.950
Unit cell angles	90.00, 95.55, 90.00

Refinement procedure

Resolution	8.000 - 1.780
R-factor	0.173 *
R-free	0.24200 *
RMSD bond length	0.009 *
RMSD bond angle	0.032
Data reduction software	MARXDS
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.800 *
High resolution limit [Å]	1.780	1.780 *
Rmerge	0.043
Total number of observations	88603 *
Number of reflections	25718
Completeness [%]	92.0	79.2 *
Redundancy	3.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18 (mg/ml)
2	1	drop		10 (mM)
3	1	drop	ammonium sulfate	25 (%)
4	1	drop	Tris-HCl	50 (mM)
5	1	reservoir	ammonium sulfate	50 (%)