1AXC

HUMAN PCNA

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Collection date	1996-10
Spacegroup name	P 32 2 1
Unit cell lengths	83.500, 83.500, 233.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.600
R-factor	0.192
Rwork	0.192
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	YEAST PCNA
RMSD bond length	0.008
RMSD bond angle	1.400
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.600
Rmerge	0.073	0.278 *
Total number of observations	130418 *
Number of reflections	33552
Completeness [%]	96.0	96.4 *
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	spermine	1 (M)
2	1	reservoir	sodium citrate	100 (mM)
3	1	reservoir	MPD	30 (%(v/v))
4	1	reservoir	sodium/potassium phosphate	200 (mM)