1AX2
ERYTHRINA CORALLODENDRON LECTIN IN COMPLEX WITH N-ACETYLLACTOSAMINE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-09 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.930, 73.170, 71.310 |
| Unit cell angles | 90.00, 113.36, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.950 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.17700 |
| Structure solution method | DIFFERENCE FOURIER FROM PREVIOUSLY DETERMINED, RELATED STRUCTURE |
| Starting model (for MR) | COMPLEX WITH LACTOSE PDB ENTRY 1LTE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 27.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.040 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.052 | 0.267 * |
| Total number of observations | 65763 * | |
| Number of reflections | 27182 | |
| <I/σ(I)> | 13 | 3.5 |
| Completeness [%] | 94.0 | 82 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | MPD | 15 (%(v/v)) | |
| 3 | 1 | drop | HEPES | 50 (mM) | |
| 4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 5 | 1 | reservoir | MPD | 30 (%) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | |
| 7 | 1 | reservoir | sodium azide | 0.02 (%) |
