1AWV

CYPA COMPLEXED WITH HVGPIA

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-02
Detector	MARRESEARCH
Spacegroup name	P 41
Unit cell lengths	74.000, 74.000, 190.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.340
R-factor	0.358
Rwork	0.358
R-free	0.46000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cyh
RMSD bond length	0.010
RMSD bond angle	25.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.380
High resolution limit [Å]	2.340	2.340
Rmerge	0.110 *	0.294 *
Total number of observations	284842 *
Number of reflections	42633
<I/σ(I)>	4.7
Completeness [%]	97.7	80.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.4	21 *	pH 8.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	peptide	6 (mg/ml)
2	1	2	ammonium sulfate	44-54 (%sat)
3	1	2	Tris-HCl	100 (mM)