1AWI

HUMAN PLATELET PROFILIN COMPLEXED WITH THE L-PRO10 PEPTIDE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-05-01
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 21 21 2
Unit cell lengths	68.250, 97.640, 39.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.204
Rwork	0.204
R-free	0.30500
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.497
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES (X-PLOR)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.350
High resolution limit [Å]	2.200	2.200
Rmerge	0.053	0.053
Number of reflections	13062
Completeness [%]	94.3	54.2
Redundancy	4.46	3.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		PROTEIN CRYSTALLIZED IN 2.0 MOLAR AMMONIUM SULFATE 0.1 MOLAR TRIS PH 8.0