1AW5
5-AMINOLEVULINATE DEHYDRATASE FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09 |
Detector | MARRESEARCH |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 102.500, 102.500, 168.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.300 |
R-factor | 0.198 |
R-free | 0.27000 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 0.023 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | MLPHARE |
Refinement software | RESTRAIN |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.600 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.088 | 0.178 |
Number of reflections | 459575 | |
<I/σ(I)> | 6.6 | 3.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 16.8 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | ENZYME CONCENTRATION 1 MG/ML, PH 7.0 - 8.5, BUFFER 0.2 M TRIS-HCL, PRECIPITANT PEG 6000 (<10%), 70 MICROMOLAR ZINC SULPHATE, 6 MM BETA-MERCAPTOETHANOL, HANGING DROPS., pH 8.0, vapor diffusion - hanging drop |