1AW5

5-AMINOLEVULINATE DEHYDRATASE FROM SACCHAROMYCES CEREVISIAE

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-09
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	102.500, 102.500, 168.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.198
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	0.023
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	MLPHARE
Refinement software	RESTRAIN

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.600	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.088	0.178
Number of reflections	459575
<I/σ(I)>	6.6	3.9
Completeness [%]	99.9	100
Redundancy	16.8	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8		ENZYME CONCENTRATION 1 MG/ML, PH 7.0 - 8.5, BUFFER 0.2 M TRIS-HCL, PRECIPITANT PEG 6000 (<10%), 70 MICROMOLAR ZINC SULPHATE, 6 MM BETA-MERCAPTOETHANOL, HANGING DROPS., pH 8.0, vapor diffusion - hanging drop