1AVY

FIBRITIN DELETION MUTANT M (BACTERIOPHAGE T4)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-09-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 3
Unit cell lengths	43.680, 43.680, 90.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.000 - 1.850
R-factor	0.22
Rwork	0.220
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aa0
RMSD bond length	0.006
RMSD bond angle	24.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	1.910
High resolution limit [Å]	1.850	1.850
Rmerge	0.036 *	0.182 *
Total number of observations	39783 *
Number of reflections	14861
Completeness [%]	89.8	53.6
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		HANGING DROPS WITH 20MG/ML PROTEIN AND 1.75M LI2SO4, 0.1M TRIS-HCL, PH7.5, AS PRECIPITANT, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Na phosphate	5 (mM)
3	1	drop		0.875 (M)
4	1	drop	Tris-HCl	0.05 (M)
5	1	reservoir		1.75 (M)
6	1	reservoir	Tris-HCl	0.1 (M)