1AVF
ACTIVATION INTERMEDIATE 2 OF HUMAN GASTRICSIN FROM HUMAN STOMACH
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG |
Synchrotron site | EMBL/DESY, HAMBURG |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.260, 50.410, 125.180 |
Unit cell angles | 90.00, 117.62, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.360 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1htr |
RMSD bond length | 0.011 |
RMSD bond angle | 26.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.400 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.066 | 0.045 |
Number of reflections | 34523 | |
<I/σ(I)> | 9.2 | 3 |
Completeness [%] | 95.5 | 80.8 |
Redundancy | 4.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.8 | PROTEIN WAS CRYSTALLIZED IN 4M NA FORMATE, 100 MM BIS-TRIS-PROPANE, PH 7.8. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium formate | 4 (M) | |
2 | 1 | reservoir | Bis-Tris propane | 100 (mM) |