1AVD

THREE-DIMENSIONAL STRUCTURE OF THE TETRAGONAL CRYSTAL FORM OF EGG-WHITE AVIDIN IN ITS FUNCTIONAL COMPLEX WITH BIOTIN AT 2.7 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	80.150, 80.150, 85.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.700
R-factor	0.174
RMSD bond length	0.019
RMSD bond angle	2.930
Refinement software	TNT

Data quality characteristics

	Overall
Rmerge	0.129 *
Total number of observations	69005 *
Number of reflections	8087 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	10 (mg/ml)
2	1	drop	phosphate	0.05 (M)
3	1	drop	ammonium sulfate	2.0 (M)
4	1	reservoir	ammonium sulfate	2.5 (M)