1AVB
ARCELIN-1 FROM PHASEOLUS VULGARIS L
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.650, 92.570, 67.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.710 - 1.900 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24200 |
| Structure solution method | MIR, DENSITY MODIFICATION, MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1loe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.157 |
| Number of reflections | 41612 | |
| <I/σ(I)> | 11.9 | 7.5 |
| Completeness [%] | 97.7 | 93.9 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.8 | 4 * | Mourey, L., (1997) Proteins: Struct.,Funct., Genet., 29, 433. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 25 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 300 (mM) | |
| 4 | 1 | reservoir | MES | 100 (mM) | pH4.8 or 4.9 |
