1AUP
GLUTAMATE DEHYDROGENASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10-12 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 162.900, 162.900, 100.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.000 - 2.500 |
| Rwork | 0.190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CLOSTRIDIUM SYMBIOSUM GLUTAMATE DEHYDROGENASE (WILD TYPE) CRYSTALLIZED WITH SODIUM GLUTAMATE IN SPACE GROUP R32. ALL WATERS AND THE BOUND GLUTAMATE SUBSTRATE REMOVED. |
| RMSD bond length | 0.017 |
| RMSD bond angle | 18.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | TFFC |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 * | 0.310 |
| Total number of observations | 90734 * | |
| Number of reflections | 17791 | |
| <I/σ(I)> | 9.5 | 2.4 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.3 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium glutamate | 1.0 (M) | |
| 3 | 1 | drop | potassium phosphate | 0.1 (M) | |
| 4 | 1 | reservoir | potassium phosphate | 0.1 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 38-46 (%) |
