1AUO
CARBOXYLESTERASE FROM PSEUDOMONAS FLUORESCENS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-03 |
| Detector | FUJI |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 82.040, 82.040, 145.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.27300 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.200 |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.071 |
| Number of reflections | 41703 |
| Completeness [%] | 91.0 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 0.10 (M) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 3 | 1 | reservoir | lithium sulfate | 0.20 (M) | |
| 4 | 1 | reservoir | dioxane | 4.0 (%(v/v)) |
