1AUO
CARBOXYLESTERASE FROM PSEUDOMONAS FLUORESCENS
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1992-03 |
Detector | FUJI |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 82.040, 82.040, 145.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.27300 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.200 |
Data reduction software | WEIS |
Data scaling software | WEIS |
Phasing software | X-PLOR (3.843) |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.071 |
Number of reflections | 41703 |
Completeness [%] | 91.0 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 0.10 (M) | |
2 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
3 | 1 | reservoir | lithium sulfate | 0.20 (M) | |
4 | 1 | reservoir | dioxane | 4.0 (%(v/v)) |