1AUC
HUMAN THIOREDOXIN (OXIDIZED WITH DIAMIDE)
Experimental procedure
| Temperature [K] | 296 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1997-05 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.750, 26.290, 51.620 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 26.000 - 2.100 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.27000 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.040 * |
| Data reduction software | MADNES |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | |
| High resolution limit [Å] | 2.100 | |
| Rmerge | 0.027 * | |
| Total number of observations | 8553 * | |
| Number of reflections | 5391 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 98.2 | 94.4 * |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | diamide | 7.25 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | DTT | 2.5 (mM) | |
| 5 | 1 | drop | PEG4000 | 9 (%) | |
| 6 | 1 | reservoir | PEG4000 | 18 (%) | |
| 7 | 1 | reservoir | 200 (mM) |
