1ATT
CRYSTAL STRUCTURE OF CLEAVED BOVINE ANTITHROMBIN III AT 3.2 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.300, 91.300, 383.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.200 |
R-factor | 0.212 |
Rwork | 0.212 |
RMSD bond length | 0.015 |
RMSD bond angle | 3.600 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.000 * |
High resolution limit [Å] | 3.200 * |
Rmerge | 0.091 * |
Total number of observations | 61000 * |
Number of reflections | 23436 * |
Completeness [%] | 84.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 13 (mg/ml) | |
2 | 1 | 2 | ammonium sulphate | 70 (%sat) | |
3 | 1 | 2 | potassium | 30 (mM) | |
4 | 1 | 2 | 1 (mM) | can be replaced with HgCl2, ZnCl2 | |
5 | 1 | 2 | 0.01 (%(w/v)) |