1ATG
AZOTOBACTER VINELANDII PERIPLASMIC MOLYBDATE-BINDING PROTEIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.934, 88.802, 41.746 |
Unit cell angles | 90.00, 93.55, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.200 |
R-factor | 0.164 * |
Rwork | 0.164 |
R-free | 0.18354 * |
Structure solution method | SINGLE ISOMORPHOUS REPLACEMENT WITH ANOMALOUS SCATTERING |
RMSD bond length | 0.007 |
RMSD bond angle | 0.020 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 ((MLPHARE) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.094 | 0.161 |
Number of reflections | 74061 | |
<I/σ(I)> | 12.7 | 6.9 |
Completeness [%] | 99.4 | 99.1 |
Redundancy | 3.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 18 * | HANGING DROP VAPOUR DIFFUSION WITH PROTEIN CONCENTRATION IN THE RANGE 10-15 MG/ML IN 10MM TRIS-HCL PH 7.0. 1 OR 2 MICROLITER DROPS OF THE LATTER WERE MIXED WITH AN EQUAL VOLUME OF 11-14% (W/V) PEG 4000 AND 0.4M AMMONIUM SULFATE IN 0.1M ACETATE BUFFER AT PH 4.0, AND THEN EQUILIBRATED AGAINST THIS SOLUTION AT 18 DEG C. CRYOPROTECTED USING THE SAME SOLUTION CONTAINING 25% (V/V) ETHYLENE GLYCOL., vapor diffusion - hanging drop, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | sodium molybdate | 1 (mM) | can be replaced by 1mM sodium tungstate |
4 | 1 | reservoir | PEG4000 | 11-14 (%(w/v)) | |
5 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
6 | 1 | reservoir | sodium acetate | 0.1 (M) |