1ASW

AVIAN SARCOMA VIRUS INTEGRASE CATALYTIC CORE DOMAIN CRYSTALLIZED FROM 20% PEG 4000, 10% ISOPROPANOL, HEPES PH 7.5 USING SELENOMETHIONINE SUBSTITUTED PROTEIN; DATA COLLECTED AT-165 DEGREES C

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	108
Detector technology	CCD
Collection date	1995-02-10
Detector	CUSTOM-MADE
Wavelength(s)	0.9464, 0.9792, 0.9790
Spacegroup name	P 43 21 2
Unit cell lengths	65.400, 65.400, 80.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.139
R-free	0.20800
RMSD bond length	0.013
RMSD bond angle	0.047
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.800
Rmerge	0.068
Number of reflections	12157
Completeness [%]	94.2
Redundancy	7.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%(w/v))
2	1	reservoir	isopropanol	10 (%)
3	1	reservoir	HEPES	100 (mM)
4	1	drop	protein	6-7.5 (mg/ml)