1ASS

APICAL DOMAIN OF THE CHAPERONIN FROM THERMOPLASMA ACIDOPHILUM

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-04-14
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	82.350, 82.350, 77.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.222
Rwork	0.222
R-free	0.25100
Structure solution method	MIR
RMSD bond length	0.017
RMSD bond angle	25.704 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.256
Total number of observations	44064 *
Number of reflections	10935
<I/σ(I)>	24.3	7
Completeness [%]	96.3	95.5
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	3.8 *	30 *	pH 3.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	27 (mg/ml)
2	1	drop	ammonium sulfate	3.0 (M)
3	1	drop	sodium citrate	0.1 (M)