1ASN

CRYSTAL STRUCTURES OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE IN TWO CONFORMATIONS: COMPARISON OF AN UNLIGANDED OPEN AND TWO LIGANDED CLOSED FORMS

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	87.600, 79.800, 89.600
Unit cell angles	90.00, 119.10, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.1893
Rwork	0.189
RMSD bond length	0.017
RMSD bond angle	2.142
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.049 *
Number of reflections	35059 *
Completeness [%]	94.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			referred to 'Smith, D. L.', (1986) J. Mol. Biol., 191, 301-302 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	sodium phosphate	0.025 (M)
3	1	reservoir	PLP	0.02 (mM)
4	1	reservoir	ammonium sulfate	50 (%sat)