1ASK
NUCLEAR TRANSPORT FACTOR 2 (NTF2) H66A MUTANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.000, 57.460, 87.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2 * |
| Rwork | 0.200 |
| R-free | 0.24285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OUN (NTF2) |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 2.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.087 | 0.464 |
| Number of reflections | 10657 | |
| <I/σ(I)> | 6.5 | 2.7 |
| Completeness [%] | 96.2 | 91.5 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.5 | Kent, H.M., (1996) J. Struct. Biol., 116, 325. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 2.5 (mM) | |
| 3 | 1 | drop | sodium acetate | 50 (mM) | |
| 4 | 1 | drop | PEG8000 | 5 (%) | |
| 5 | 1 | drop | sodium azide | 1 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 10 (%) | |
| 8 | 1 | reservoir | sodium azide | 2 (mM) |
