1ASJ

P1/MAHONEY POLIOVIRUS, AT CRYOGENIC TEMPERATURE

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	113
Detector technology	AREA DETECTOR
Collection date	1995-12
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	320.150, 355.550, 377.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.000 - 2.900
R-factor	0.253 *
Rwork	0.253
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2plv
RMSD bond length	0.014
RMSD bond angle	26.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.900
Rmerge	0.121
Total number of observations	1028010 *
Number of reflections	421914
Completeness [%]	45.0
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5	4 *	VIRUS WAS CRYSTALLIZED BY MICRODIALYSIS AGAINST 10MM PIPES, 0-70 MM NACL, PH 7.5, 2% PEG 400, microdiaylsis

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		70 (mM)
2	1	1	PEG400	2 (%)