1AS3

GDP BOUND G42V GIA1

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	110
Detector technology	CCD
Collection date	1996-09
Spacegroup name	I 4
Unit cell lengths	121.440, 121.440, 68.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.212
Rwork	0.212
R-free	0.27400
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1gdd
RMSD bond length	0.011
RMSD bond angle	23.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.063	0.330
Number of reflections	19384
<I/σ(I)>	20.5
Completeness [%]	99.3	99.2
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6 *	20 *	THE PROTEIN WAS CRYSTALLIZED IN HANGING DROPS USING 4M (NH4)2SO3 AS THE PRECIPITANT. 50 MM HEPES, PH 8.0, 10 MM MGSO4, 10 MM DTT AND 5 MM GDP WAS THE BUFFER., vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-7.5 (mg/ml)
2	1	drop		0.75-1.0 (M)
3	1	drop	sodium acetate	50 (mM)
4	1	reservoir		1.5-2.0 (M)
5	1	reservoir	sodium acetate	100 (mM)