1ARZ
ESCHERICHIA COLI DIHYDRODIPICOLINATE REDUCTASE IN COMPLEX WITH NADH AND 2,6 PYRIDINE DICARBOXYLATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 294 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-08 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 137.600, 123.800, 66.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dih |
| RMSD bond length | 0.016 |
| RMSD bond angle | 25.000 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.081 * | 0.269 * |
| Total number of observations | 119624 * | |
| Number of reflections | 32245 | 4365 * |
| <I/σ(I)> | 15.9 | 2 |
| Completeness [%] | 90.0 | 74 |
| Redundancy | 3.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | PROTEIN WAS CRYSTALLIZED FROM 23-26% PEG 8000, IN 160-180 MM POTASSIUM PHOSPHATE, 100 MM SODIUM CACODYLATE PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 23-26 (%) | |
| 2 | 1 | reservoir | potassium phosphate | 160-180 (mM) | |
| 3 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | drop | NADH | 2 (mM) | |
| 6 | 1 | drop | PDC | 30 (mM) |
