1ART
X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAL 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN OPEN AND CLOSED FORM
Experimental procedure
Spacegroup name | C 2 2 21 |
Unit cell lengths | 157.140, 85.510, 78.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 * - 1.800 |
R-factor | 0.217 |
Rwork | 0.217 |
RMSD bond length | 0.017 |
RMSD bond angle | 3.300 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.045 * |
Total number of observations | 105688 * |
Number of reflections | 31930 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 0.9 (mM) | |
10 | 1 | reservoir | sodium sulfate | 1 (M) | |
2 | 1 | drop | potassium phosphate | 10 (mM) | |
3 | 1 | drop | PLP | 0.01 (mM) | |
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | 0.3 (mM) | ||
6 | 1 | reservoir | potassium phosphate | 10 (mM) | |
7 | 1 | reservoir | PLP | 0.01 (mM) | |
8 | 1 | reservoir | EDTA | 1 (mM) | |
9 | 1 | reservoir | 0.3 (mM) |