1ARP

Crystal structure of the fungal peroxidase from Arthromyces ramosus at 1.9 angstroms resolution: structural comparisons with the lignin and cytochrome C peroxidases

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	74.570, 74.570, 117.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 1.900
R-factor	0.174
Rwork	0.174
RMSD bond length	0.021
RMSD bond angle	0.058 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.058 *
Total number of observations	83158 *
Number of reflections	19789 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	24 *	used as seeds, referred to 'Kunishima, N.', (1993) 'Proteins.Struct.,Funct., Genet.', 15, 216-220 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ARP	20-40 (mg/ml)
2	1	reservoir	ammonium sulfate	32 (%sat)
3	1	reservoir	Tris-HCl	20 (mM)