1AR7
P1/MAHONEY POLIOVIRUS, DOUBLE MUTANT P1095S + H2142Y
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 113 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 319.950, 355.100, 377.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.000 - 2.900 |
| Rwork | 0.234 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ar9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.580 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.120 |
| Total number of observations | 2373483 * |
| Number of reflections | 770521 |
| Completeness [%] | 82.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 | 4 * | VIRUS WAS CRYSTALLIZED BY MICRODIALYSIS AGAINST 10MM PIPES, 0-70 MM NACL, PH 7.5, 2% PEG 400, microdiaylsis |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 70 (mM) | ||
| 2 | 1 | 1 | PEG400 | 2 (%) |
