1AR2
DISULFIDE-FREE IMMUNOGLOBULIN FRAGMENT
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1996-08-28 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 52.800, 52.800, 158.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.000 - 2.800 |
R-factor | 0.228 * |
R-free | 0.34300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rei |
RMSD bond length | 0.004 |
RMSD bond angle | 30.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXL-97 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.104 | 0.376 |
Total number of observations | 37985 * | |
Number of reflections | 3549 | |
<I/σ(I)> | 11.1 | 2.7 |
Completeness [%] | 97.0 | 97.8 |
Redundancy | 10.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 4 * | VAPOUR DIFFUSION AL 4 C FROM A PROTEIN SOLUTION CONTAINING 8 MG/ML IN 50 MM POTASSIUM PHOSPHATE BUFFER AL PH 7, USING 2 M AMMONIUM SULFATE IN 100 MM SODIUM ACETATE BUFFER, PH 4.6, vapor diffusion, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 50 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 2 (M) | |
4 | 1 | reservoir | sodium acetatex | 100 (mM) |