1AQZ

CRYSTAL STRUCTURE OF A HIGHLY SPECIFIC ASPERGILLUS RIBOTOXIN, RESTRICTOCIN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-08
Detector	FUJI
Wavelength(s)	0.7, 2.05
Spacegroup name	P 1 21 1
Unit cell lengths	50.240, 82.160, 38.040
Unit cell angles	90.00, 100.50, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.177 *
Rwork	0.237
R-free	0.23700 *
Structure solution method	SINGLE ISOMORPHOUS REPLACEMENT AND ANOMALOUS SCATTERING
RMSD bond length	0.018
RMSD bond angle	27.100 *
Data reduction software	LAUEVIEW
Data scaling software	LAUEVIEW
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	1.780
High resolution limit [Å]	1.700	1.700
Rmerge	0.039
Number of reflections	28328
Completeness [%]	85.6	54.8
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8		A COMBINATION OF VAPOR DIFFUSION AND MICRODIALYSIS TECHNIQUES WERE USED TO CRYSTALLIZE RESTRICTOCIN. THE FINAL MOTHER LIQUOR CONSISTS OF 60% ETHANOL, 10MM SODIUM PHOSPHATE, PH6.8. THE PROTEIN CONCENTRATION IS 10MG/ML., vapor diffusion and microdialysis

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ethanol	60 (%)
2	1	reservoir	sodium phosphate	10 (mM)
3	1	drop	protein	10 (mg/ml)