1AQT

EPSILON SUBUNIT OF F1F0-ATP SYNTHASE FROM ESCHERICHIA COLI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-09
Detector	RIGAKU RAXIS II
Spacegroup name	P 65 2 2
Unit cell lengths	94.610, 94.610, 56.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.000 - 2.300
R-factor	0.214
Rwork	0.214
R-free	0.28800
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.059 *	0.475 *
Total number of observations	19833 *
Number of reflections	6626
<I/σ(I)>	12.4	1.8
Completeness [%]	94.0	89
Redundancy	3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		PROTEIN (4 MG/ML)IN 50 MM HEPES BUFFER, PH 7.5, 200 MM (NH2)2SO4, 2 M (NA/K)PO4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		2 (M)
2	1	reservoir	HEPES	100 (mM)
3	1	reservoir		200 (mM)
4	1	drop	protein	2 (mg/ml)
5	1	drop		1 (M)
6	1	drop	HEPES	50 (mM)
7	1	drop		100 (mM)